Publications

A complete publication listing can be found on Pubmed. See select publications below.

Jak2V617F Reversible Activation Shows an Essential Requirement for Jak2V617F in Myeloproliferative Neoplasms

Published in Preprint-biorxiv, 2022

In this manuscript we developed a reversible JAK2V617F model with Dre->Cre functionality, such that the Cre sites which delete/reverse JAK2V617F are only available after Dre recombination induces mutant expression. This allowed us to test the dependency of JAK2-mutations in propagating myeloproliferative disease.

Recommended citation: Andrew Dunbar, Robert L. Bowman, Young Park, Franco Izzo, Robert M. Myers, Abdul Karzai, Won Jun Kim, Inés Fernández Maestre, Michael R. Waarts, Abbas Nazir, Wenbin Xiao, Max Brodsky, Mirko Farina, Louise Cai, Sheng F. Cai, Benjamin Wang, Wenbin An, Julie L Yang, Shoron Mowla, Shira E. Eisman, Tanmay Mishra, Remie Houston, Emily Guzzardi, Anthony R. Martinez Benitez, Aaron Viny, Richard Koche, Dan A. Landau, Ross L. Levine. Jak2V617F Reversible Activation Shows an Essential Requirement for Jak2V617F in Myeloproliferative Neoplasm. bioRxiv 2022.05.18.492332; doi: https://doi.org/10.1101/2022.05.18.492332

Modeling clonal evolution and oncogenic dependency in vivo in the context of hematopoietic transformation

Published in Preprint-biorxiv, 2022

In this manuscript we develop tools for inducing up to three sequential mutations in mice for model the evolution from clonal hematopoiesis to acute myeloid leukemia. In order to investigate oncogene dependency, further develop tools to turn on a mutant oncogene, and subsequently revert that mutation.

Recommended citation: Robert L. Bowman, Andrew Dunbar, Tanmay Mishra, Wenbin Xiao, Michael R. Waarts, Inés Fernández Maestre, Shira E. Eisman, Louise Cai, Sheng F. Cai, Pablo Sanchez Vela, Shoron Mowla, Anthony R. Martinez Benitez, Young Park, Isabelle S. Csete, Aishwarya Krishnan, Darren Lee, Nayla Boorady, Chad R. Potts, Matthew T. Jenkins, Martin P. Carroll, Sara E. Meyer, Linde A. Miles, P. Brent Ferrell Jr., Jennifer J. Trowbridge, Ross L. Levine. Modeling clonal evolution and oncogenic dependency in vivo in the context of hematopoietic transformation. bioRxiv 2022.05.18.492524; doi: https://doi.org/10.1101/2022.05.18.492524

Single-cell mutation analysis of clonal evolution in myeloid malignancies

Published in Nature, 2020

In this manuscript we utilize single cell DNA-sequencing to evaluate clonal abundance and mutation order in patient samples ranging from clonal hematopoiesis to relapsed/refractory leukemia. We combined scDNA-seq with oligonucleotide conjugated antibodies to resolve how genotype influences differentiation/immunophenotype.

Recommended citation: Miles LA*, Bowman RL*, Merlinsky TR, Csete IS, Ooi AT, Durruthy-Durruthy R, Bowman M, Famulare C, Patel MA, Mendez P, Ainali C, Demaree B, Delley CL, Abate AR, Manivannan M, Sahu S, Goldberg AD, Bolton KL, Zehir A, Rampal R, Carroll MP, Meyer SE, Viny AD, Levine RL. "Single-cell mutation analysis of clonal evolution in myeloid malignancies." Nature. 2020 Nov;587(7834):477-482.

Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation.

Published in Cell Stem Cell, 2019

Here we investiagated the role of Stag2 and Stag1 knockouts on hematopoietic function.

Recommended citation: Viny AD, Bowman RL, Liu Y, Lavallée VP, Eisman SE, Xiao W, Durham BH, Navitski A, Park J, Braunstein S, Alija B, Karzai A, Csete IS, Witkin M, Azizi E, Baslan T, Ott CJ, Peer D, Dekker J, Koche R, Levine RL. "Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation." Cell Stem Cell. 2019 Nov 7;25(5):682-696.e8.

Clonal Hematopoiesis and Evolution to Hematopoietic Malignancies.

Published in Cell Stem Cell, 2018

Here we reviewed the mutations associatd with clonal hematopoiesis, how they influence evolution to malignancy, and how mutant clones non hematological disease progression.

Recommended citation: Bowman RL, Busque L, Levine RL. Clonal Hematopoiesis and Evolution to Hematopoietic Malignancies." Cell Stem Cell. 2018 Feb 1;22(2):157-170.